Pyrolysis-GCxGC-QTOF for improved characterization of crude oils
Crude oil consists of an extremely large amount of compounds covering a wide range of volatility and chemistry. This makes detailed composition characterization highly challenging.
Pyrolysis coupled to comprehensive two-dimensional gas chromatography-high resolution mass spectrometry (py-GC×GC-HRMS) is a very powerful technique for the characterization of complex, heavy matrices such as crude oils. The two-dimensional resolution provides enhanced separation of the pyrolysis products, leading to improved classification for groups and individual analytes. Additionally, the 2D pyrograms make sample comparison easier and more informative. In addition, high resolution and accurate mass can deliver extra selectivity and identification power, especially for compounds with heteroatoms (e.g. N, S, O).
Here we show the use of py-GC×GC for improved characterization and comparison of crude oils and the advantages arising from HRMS detection for speciation of heteroatoms such as sulfur-containing compounds.
New app note about the profiling of gin botanicals by headspace-SPME Arrow-GCxGC-MSD/FID
GCxGC-MS is the ideal analytical technique to accommodate the complexity of samples, as in this case gins, due to its enhanced peak capacity and sensitivity. For quantitative purposes, and when using flow modulation, splitting to a FID is a logical choice. Sample preparation and sample introduction introduce further challenges due to the complex nature of the samples. Headspace-SPME Arrow is a fully automated sample preparation technique with the ability to extract targeted key compounds present a low concentrations whilst avoiding matrix compounds present at high concentrations. The combination of HSSPME Arrow with GCxGC-MSD/FID is a very powerful tool in the comparative analysis of samples with complex matrices.
Comparing two chromatograms side-by-side with GC Image version 2.8R1
Comparing two chromatograms side-by-side with GC Image version 2.8R1
At the GCxGC Symposium 2018, GC Image presented their new Compare Images – Side-by-side tool that allows comprehensive peak matching between two chromatograms, and automates difference detection. The tool is included in the latest GC Image release, Version 2.8R1.
To view the poster or download a trial version to test new features, click on the link.
The JSB Automation Challenge!
JSB UK & Ireland are now accepting automation challenges! Do you have a laborious, time-consuming or difficult sample preparation? Are you dealing with unpleasant or hazardous reagents? Why not automate your sample preparation?!
If you are interested in seeing your sample preparation procedure automated, simply submit your manual sample preparation procedure to email@example.com in confidence with the heading 'Automation Challenge', and we will present you with a video of your automated solution.
Challenge us! See the videos on the links below as some examples of what we can do, from simple to complex sample preparation to full automation with intelligent software.
Visit us on our stand and enjoy a cocktail prepared by our robot barman!
PVC plasticizer in food and toys
CTC published a new GC/MS application note about the fully automated sample preparation for the determination of plasticizers in PVC from food contact materials and toys.
Food contact materials (FCM) made from PVC, such as e.g. gaskets of metal lids or cling films, may release plasticizers into the packed food. Such migration repeatedly exceeded legal limits or non-authorized plasticizers have been used. In a European enforcement campaign on migration from gaskets into oily foods in 2011, for example, legal limits were exceeded in 24% of the 308 samples analyzed. The EU also banned the use of certain phthalates as plasticizers for toys and childcare products, whereas its content is limited to below 0.1 %. Furthermore the directive 2011/65/EU will restrict “certain hazardous substances in electrical and electronic equipment”, including the same group of phthalates. Plasticizers are analyzed and quantified by GC-FID or MS, if detection limits lower than 0.1 % are required. So far up to 40 different plasticizers were found and quantified in FCM and toys.
GCxGC with parallel MS and FID detection
Our application specialist Daniela Peroni published a new application note:
GCxGC with parallel MS and FID detection for improved analysis of fragrances.
Gas chromatography Mass Spectrometry (GC-MS) is broadly employed for the analysis of allergens and aroma compounds in fragrances. However, the analytical challenge is not trivial. The complexity of essential oils and perfumes typically requires multiple analyses with different stationary phases to achieve suitable resolution for all targets.
Comprehensive two-dimensional gas chromatography (GC×GC) couples two columns in a single analysis to achieve superior separation power. Peaks are distributed across a 2D separation space based on different physicochemical properties, enhancing resolution and significantly reducing co-elution. These advantages make GC×GC-MS increasingly more widespread in the fragrance industry for accurate and time-effective targeted analysis as well as untergated profiling.
Here we demonstrate the feasibility of thermally-modulated GC×GC with parallel MS/FID detection to combine enhanced identification capability and robust, reliable quantification in fragrance analysis.
GC IMAGE ANNOUNCED THE RELEASE OF VERSION 2.9 (V2.9R1)
GC Image announced the release of Version 2.9 (v2.9r1) for GC Image and LC image. This latest version includes new features, improvements, and critical bug fixes over 2.8 releases. As usual, this latest release has been evaluated by JSB and we created 2 documents for your convenience to give you an overview in a glance. The first document is a historical overview of new features introduced since the release of version 2.7r1 until this last release and the second document is a summary of just this release compared to the previous version.
Click on the link for more information or have a read in the documents.
Coupling GCxGC with QTOF detection in MS/MS Mode
Coupling GCxGC with QTOF detection in MS/MS mode: a feasibility study
Our application specialist Daniela Peroni has published a new application note about Coupling GCxGC with QTOF detection in MS/MS mode.
The Agilent 7250 QTOF detector delivers high-resolution MS data, tunable ionization energy and MS/MS capability. Accurate-mass spectra with classic ionization can therefore be complemented with soft ionization spectra as well as MS/MS data for identity confirmation of targets and structure elucidation of unknowns. This makes GC-QTOF valuable for challenging applications that demand confident identification in highly complex matrices (e.g. metabolomics, pesticides analysis).
Comprehensive two-dimensional gas chromatography (GC×GC) is the chromatographic method of choice to unravel high sample complexity. Compared to classic GC, GC×GC grants superior separation power and unmatched peak capacity by coupling two different separation mechanism in a single analysis. Co-elutions are avoided or minimized, granting easier identification and more reliable quantification.
Several examples are available for GC×GC in combination with the QTOF used in scan mode as a HRMS-TOF detector. The elevated number of resolved peaks and the clean 2D spectra, in combination with formula generation based on accurate-mass data, allow more precise and informative profiling. However, up to now GC×GC is hardly used in combination with the QTOF in MS/MS mode. In the full application note the feasibility of GC×GC-QTOF-MS/MS is investigated.