Microtissue-based screening using automation and the EnSight multimode plate reader to generate and characterize scaffold-free 3D cell cultures
Stuart McLarnon, Application Specialist, PerkinElmer
Three-dimensional (3D) cell culture spans the gap between the traditional “petri-dish-based” format and whole-animal systems giving unique perspectives on tumorigenesis. Process standardization is essential to enable the generation of robust and reproducible data with more biologically relevant, complex, 3D models.
Production and characterization of 3D spheroid microtissues can be simplified and standardized by integrating application specific microplates, automated liquid handlers and imaging enabled multimode plate readers which combine to reduce variability, hands-on time and errors produced by manual pipetting. Microtissue growth can be assessed directly using the imaging module of the EnSight plate reader and a custom-developed analysis method that automatically generates brightfield images of each well, identifies and measures spheroid area. This combined technology approach allows for rapid monitoring of tumour microtissue growth without necessitating the addition of a fluorescent label.